Isolation of Antimicrobial-Resistant Enterobacteriaceae and Nonfermenting Bacteria From Livestock-Associated Ambient Particulate Matter
The issue of antimicrobial resistance, particularly among Gram-negative enteric bacteria, in agricultural settings has been documented in literature and continues to be a growing public health concern. Among the Enterobacteriaceae family, the presence of extended spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Enterobacteriaceae (CRE) are especially concerning because of the impact they have on healthcare worldwide. We employed a novel approach by constructing and placing adhesive air strips in a stockyard in Madison County, Kentucky. Samples were placed in enteric enrichment (EE) broth after collection and incubated for 24 hr. Samples that turned yellow due to fermentation were subsequently inoculated on ESBL and CRE screening media. We transferred colonies that grew on both plates onto MacConkey and sheep blood agar to use for further testing. We recovered 8 of the 10 placed air strips. Of the EE broth tubes, 7 of the 16 turned yellow, indicative of enteric bacteria. Of the 7 positive tubes, 5 isolates grew on ESBL and CRE screening media with only 1 isolate displaying pigmentation indicative of a resistant organism. We were able to isolate resistant enteric bacteria from particulate matter in an agricultural setting using our novel sampling approach.
Speaker / Author:
Clint Pinion, Jr., MA, DrPH, RS, CIT, Division of Health Technologies, Southwest Virginia Community College
S. Travis Altheide, PhD, MLS (ASCP)CM, Department of Medical Laboratory Science, Eastern Kentucky University